3 hour image analysis workshop run twice yearly including some hands on practical exercises.
Content includes: basics of ImageJ/FIJI image analysis software including opening files, general image manipulation, simple thresholding, object detection/counting and basic automation.
2 hour slide scan analysis workshop run twice yearly.
Content includes: introduction to QuPath digital pathology slide analysis software, basic automated cell detection, TMA analysis, machine learning pixel classification and object detection and introduction to simple automation scripts.
Fiji is an image processing package — a “batteries-included” distribution of ImageJ, bundling many plugins which facilitate scientific image analysis. Easy to use, free and open source.
Use ZEN lite as a viewer for your CZI files or other standard file types. Perform image acquisition or fundamental image analysis and processing tasks.
CellProfiler is free, open-source software for quantitative analysis of biological images.
Open software for bioimage analysis – often used for digital pathology applications. Offers a powerful set of tools for working with whole slide images.
Understand both the basic concepts and advanced principles in optical microscopy. Reference library, online tutorials, articles and reviews.
Review important topics for understanding the microscope, such as resolution, numerical aperture, depth of field, image brightness, objective working distance, field of view, conjugate planes, and the useful magnification range. Online tutorials, techniques and articles.
Online resource centre for basic microscopy concepts, digital imaging guides, tutorials, techniques, physics of light and colour, and more.
Support for beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies.
Online tool that allows you to assess the spectral compatibility of dyes and probes; design and optimise fluorescence-based experiments for imaging, flow cytometry, or microplate reader instruments; quantitate spectral overlap; export and print designs.
FPbase is a free and open-source, community-editable database for fluorescent proteins (FPs) and their properties. Each protein in the database shows amino acid sequence, accession IDs, evolution lineages and mutations, fluorescence attributes, structural data, references and so forth.
Input variables including: