Flow staff run a monthly 2-hour ‘Introduction to Flow Cytometry’ course open to new and existing users. This covers what a flow cytometer is, and how to choose dyes, set up panels, gating and compensation. New users must attend the ‘Introduction to Flow Cytometry’ course to be able to book a hands on instrument training.
Cell analysis online support centre from Thermo Fisher Scientific – technical information, tips & tricks, and answers to common problems.
FlowJo is the leading analysis platform for single-cell flow and mass cytometry analysis. Explore online tutorials to assist with everything from getting your analysis set up, quality control and organisational tips, to more advanced tutorial topics using high parameter data.
FlowJo AutoSpill is an algorithm for calculating spillover and producing a compensation matrix to help improve compensation without well-defined positive and negative populations.
Variability in cell counting results can be due to a variety of causes – including: sample preparation, focus and light settings, and gating strategies. Thermo Fisher’s white paper outlines the 4 pillars to minimise result inconsistencies.
This online guide to fluorescent probes and labelling technologies contains over 3,000 reagents and kits representing a wide range of labelling and detection products, and features extensive references and technical notes.
FluroFinder allows you to search over 3 million antibodies online. Ideal for difficult to find antibodies.
Use to normalise the relationship between positive and negative populations to compare treated and untreated samples.
SI = (MFIpos – MFIneg ) / (2 x SDneg)
SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.